一、个人简介

1986.9 – 1991.7 南京医科大学预防医学系，学士

1993.9 – 1996.7 南京医科大学，流行病和统计学系，硕士

2002.3 – 2005.7 东南大学，生物医学工程系，博士

2008.10 - 2009 .10 德国肿瘤研究中心，研究方向：生物芯片和胰腺肿瘤干细胞

2005 – 至今 北京大学基础医学院细胞生物学系，干细胞研究中心，讲师、副教授，硕士生导师

二、研究方向：

1. 工程化细胞：细胞膜功能性受体和配体的信号调制及变换；解析人和小鼠新型单克隆抗体的结构和编码DNA序列；诱导多能干细胞（iPSC）、T细胞和NK细胞的工程化修饰，嵌合抗原受体（CAR）-T细胞的结构和序列； NK细胞膜受体和配体信号的调节，NK细胞的细胞表面功能分子的结构域变换等；

2. 基因编辑和数据分析：采用Crispr-Cas9方法在细胞水平上敲除（Knockout）或敲入(Knock-in)相关的基因；DNA质粒的构建，免疫治疗载体的设计和组装；基因敲降（Knockdown）方法和microRNA检测；基因芯片、高通量测序的基因表达数据分析；

3. 细胞代谢和纳米分子对细胞的作用：细胞内氨基酸代谢和纳米分子的转运，类酯纳米分子在细胞内聚集和代谢的分子机制，研究纳米分子与干细胞和肿瘤干细胞之间的相互作用，肿瘤细胞和肿瘤干细胞的耐药性及相应对策。

三、主持科研项目：

1. 北京市自然科学基金面上项目，编号： 5202011，氨基酸转运载体LAT1和mTOR通路对有机纳米荧光素标记细胞的影响，主持，2020/01-2022/12。

2. 国家重点研发计划，课题编号2018YFE0205303，靶向调控肿瘤免疫微环境的纳米机器人药物的设计、构筑及抗肿瘤疗效，参与骨干，2019/8-2022/8。

3. 国家自然科学基金重点项目，21233003，干细胞与荧光碳纳米材料相互作用的物理化学实验研究，参加北京师范大学化学学院范楼珍教授主持的课题，合作单位主持， 2013/01-2017/12。

4. 国家自然科学基金面上探索项目，项目名称：人胚胎干细胞表达基因HESP26的电子克隆、验证和功能研究，批准号30871254，时间2009/01-2009/12，主持。

四、专利：

1. 李凌松、周士新、文锦华。2012年作为第二发明人对这个基因和编码蛋白申请了我国的发明专利，2014年获得批准，专利号为：ZL201110159475，名称：“C9ORF135基因、其编码的蛋白及其抗体的应用”。

2. 邓宏魁、高君双、周士新。 2022 年已授权的 CAR-T 专利，名称为：一种靶向 CD19 和CD20 双抗原的嵌合性抗原受体 T 细胞治疗肿瘤的方法，专利号 ZL201810618266.0

3. 周士新、邓常文。2023 年申请发明专利1项，名称为“一种利用 CD27 逆转 T 和 CAR-T 细胞 PD1 信号的工程化细胞方法”，申请号为202311258648.4。

五、编写教材：

教育部基础医学“101计划”核心教材《医学分子细胞遗传基础》的编委，具体负责第五篇细胞和社会性与细胞命运：第28章，细胞分化。

六、代表性研究论文：

1. Meng F^#, Zhang S, Xie J, Zhou Y, Wu Q, Lu B, Zhou S*, Zhao X*, Li Y*. Leveraging CD16 fusion receptors to remodel the immune response for enhancing anti-tumor immunotherapy in iPSC-derived NK cells. J Hematol Oncol. 2023;16(1):62

2. Pan Xu^#, Shixin Zhou^#, Sergey I. Druzhinin, Holger Holger Schönherr*, Bo Song*, Design of a pH-sensitive supramolecular fluorescent probe for selective cancer cell imaging. Dyes and Pigments,2023,217,111366

3. Ke Li^#, Shixin Zhou^#, Yabei Chen, Pan Xu, Bo Song,Supramolecular fluorescence nanoprobe loaded with azobenzene for the detection of azoreductase: Selective light-up of hypoxic cells,Sensors and Actuators B: Chemical,2022,363,131860

4. Shixin Zhou^#, Jing Wu^#, Xiaotian Huang, Ning Yu, Xiajuan Zou, Hong Tang, Jastaranpreet Singh, Bo Song^*, Yang Li^*, Cellular uptake of a cationic amphiphilic fluorophore in the form of assemblies via Clathrin-dependent endocytosis, Materials & Design,2021,200,109464

5. Su W^#, Guo R^#, Yuan F, Li Y, Li X, Zhang Y^*, Zhou S^*, and Fan L^*. Red-Emissive Carbon Quantum Dots for Nuclear Drug Delivery in Cancer Stem Cells.  The Journal of Physical Chemistry Letters.  2020,  11 (4), 1357-1363

6. Zhou S^#, Deng C^#, Xu P^#, Fan Q, Zhang X, Jia Y, Su L, He Q^*, Liu Y^* and Bo S^*. Cellular Metabolism of Fluorescent Nanoprobes Formed by Self-Assembly of Amphiphiles: Dynamic Trafficking from the Golgi Apparatus to the Lysosome. ACS Applied Bio Materials. 2019;2:5790-8.

7. Xing Y^#, Li D, Dong B, Wang X, Wu C, Ding L, Zhou S^*, Fan J and Bo S^*. Water-soluble and highly emissive near-infrared nano-probes by co-assembly of ionic amphiphiles: towards application in cell imaging. New Journal of Chemistry. 2019;43:8059-66.

8. Zhou S^#, Zhao H^#, Feng R^#, Ding L, Li Z, Deng C, He Q, Liu Y^*, Song B^*, Li Y^*. Application of amphiphilic fluorophore-derived nanoparticles to provide contrast to human embryonic stem cells without affecting their pluripotency and to monitor their differentiation into neuron-like cells. Acta Biomater. 2018:78:274-284..

9. Zhou S^#, Xia Y, Liu Y, He Q, Song B^*. Aggregation induced emission fluorogens light cells via microtubules: accessing the mechanisms of intracellular trafficking of ionic substances. Langmuir. 2017;33(23):5947-5956.

10. Shixin Zhou^#, Yinan Liu, Yumin Ma, Xiaoyan Zhang, Yang Li, and Jinhua Wen^*.  C9ORF135  encodes a membrane protein whose expression is related to pluripotency in human embryonic stem cells. Scientific Reports.2017, 7: 45311

11. Shixin Zhou^#, Yinan Liu^#, Ruopeng Feng^#, Caiyun Wang, Sibo Jiang, Xiaoyan Zhang, Feng Lan^*, Yang Li^*. Survivin Improves Reprogramming Efficiency of Human Neural Progenitors by Single Molecule OCT4. Stem Cells Int. 2016; 2016: 4729535

12. Yun Bai^#, Shifeng Kan^#, Shixin Zhou^#, Yuting Wang, Jun Xu, John P Cooke^(*), Jinhua Wen^(*),Hongkui Deng^(*).Enhancement of the in vivo persistence and antitumor efficacy of CD19 chimeric antigen receptor T cells through the delivery of modified TERT mRNA. Cell Discovery. 2015, 1, 15040

13. Jin J^#, Zhou S^#, Xu Q, An J^*. Identification of risk factors in epidemiologic study based on ROC curve and network. Sci Rep. 2017,7:46655

14. Yijun Xia^#, Lin Dong, Yingzhi Jin, Shuai Wang, Li Yan, Shouchun Yin^*, Shixin Zhou^* and Bo Song^*. Water-soluble nano-fluorogens fabricated by self-assembly of bolaamphiphiles bearing AIE moieties: towards the application in cell imaging. Journal of Materials Chemistry B. 2015,3, 491-497

15. Fan Z^#, Zhou S, Garcia C, Fan L*, Zhou J*. pH-responsive Fluorescent Graphene Quantum Dots for Fluorescence-Guided Cancer Surgery and Diagnosis. Nanoscale. 2017; 9(15):4928-4933

16. Shuhua Li^#, Shixin Zhou, Yunchao Li, Xiaohong Li, Jia Zhu, Louzhen Fan*, and Shihe Yang*. Exceptionally High Payload of the IR780 Iodide on Folic Acid-Functionalized Graphene Quantum Dots for Targeted Photothermal Therapy. ACS Applied Materials & Interfaces. 2017, 9 (27), 22332-22341

17. Ruihua Guo^#, Shixin Zhou, Yunchao Li, Xiaohong Li, Louzhen Fan*, and Nicolas H. Voelcker*. Rhodamine-Functionalized Graphene Quantum Dots for Detection of Fe³⁺ in Cancer Stem Cells. ACS Appl. Mater. Interfaces 2015, 7, 23958−23966

18. Feng R^#, Zhou S, Liu Y, Song D, Luan Z, Dai X, Li Y, Tang N, Wen J*, Li L*. Sox2 protects neural stem cells from apoptosis via up-regulating survivin expression. Biochem J. 2013;450(3):459-468

19. Zhou S^#, Liu Y, Bo H, Bian X, Xia X, Lin C, Wong VW, Lu Z*. Expression Profilings of 39 Genes Selected by ANOVA Could Separate Precursors of Murine Dendritic Cells and Macrophages. Biochemical and Biophysical Research Communications. 2006; 344:438-445

20. Zhou S^#, Liu Y^#, Weng J, Kong L, Sun X, Gu B, Lu Z*. Profiles of Mouse Dendritic Cell Sarcoma Are Similar to Hematopoetic Stem Cells or Progenitors by Clustering and Principal Component Analyses. Biochemical and Biophysical Research Communications. 2005;331(1):194-202

七、联系方式：

Email : zsx@bjmu.edu.cn